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ramp proteins  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology ramp proteins
    Figure 3. Double-labelling immunocytochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,D,G) and the expression of receptor activity-modifying protein <t>(RAMP)</t> 1 (B), <t>RAMP2</t> (E), or <t>RAMP3</t> (H) in BON-1 cells. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling <t>of</t> <t>RAMP1,</t> RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,F,I). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Scale bar: 100 µm.
    Ramp Proteins, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 8 article reviews
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    Images

    1) Product Images from "Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues."

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms24043960

    Figure 3. Double-labelling immunocytochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,D,G) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (E), or RAMP3 (H) in BON-1 cells. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,F,I). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Scale bar: 100 µm.
    Figure Legend Snippet: Figure 3. Double-labelling immunocytochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,D,G) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (E), or RAMP3 (H) in BON-1 cells. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,F,I). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Scale bar: 100 µm.

    Techniques Used: Expressing, Activity Assay, Staining

    Figure 6. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pituitary tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: CALCRL-negative cells that express RAMP1 or RAMP3. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K); 50 µm (D,H,L).
    Figure Legend Snippet: Figure 6. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pituitary tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: CALCRL-negative cells that express RAMP1 or RAMP3. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K); 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 7. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human duodenal tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visu- alised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour repre- sents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: Neuroendocrine cells. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).
    Figure Legend Snippet: Figure 7. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human duodenal tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visu- alised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour repre- sents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: Neuroendocrine cells. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 8. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pancreatic tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Asterisks: Pancreatic islets. Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).
    Figure Legend Snippet: Figure 8. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pancreatic tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Asterisks: Pancreatic islets. Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 9. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human adrenocortical tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).
    Figure Legend Snippet: Figure 9. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human adrenocortical tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 10. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human placental tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Arrows: Syncytiotrophoblast cells; arrowheads: Capillary endothelia; asterisks: Non-specifically stained erythrocytes. Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).
    Figure Legend Snippet: Figure 10. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human placental tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Arrows: Syncytiotrophoblast cells; arrowheads: Capillary endothelia; asterisks: Non-specifically stained erythrocytes. Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 12. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in medullary thyroid cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour. Blue colour represents 4′,6- diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent (C,D,G,H,K,L) enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).
    Figure Legend Snippet: Figure 12. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in medullary thyroid cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour. Blue colour represents 4′,6- diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent (C,D,G,H,K,L) enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 13. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in renal clear cell cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).
    Figure Legend Snippet: Figure 13. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in renal clear cell cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Techniques Used: Immunohistochemical staining, Expressing, Activity Assay, Staining



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    Image Search Results


    Figure 3. Double-labelling immunocytochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,D,G) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (E), or RAMP3 (H) in BON-1 cells. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,F,I). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Scale bar: 100 µm.

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 3. Double-labelling immunocytochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,D,G) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (E), or RAMP3 (H) in BON-1 cells. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,F,I). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Scale bar: 100 µm.

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Expressing, Activity Assay, Staining

    Figure 6. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pituitary tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: CALCRL-negative cells that express RAMP1 or RAMP3. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K); 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 6. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pituitary tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: CALCRL-negative cells that express RAMP1 or RAMP3. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K); 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 7. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human duodenal tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visu- alised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour repre- sents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: Neuroendocrine cells. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 7. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human duodenal tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visu- alised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour repre- sents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Arrows: Neuroendocrine cells. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 8. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pancreatic tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Asterisks: Pancreatic islets. Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 8. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human pancreatic tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Asterisks: Pancreatic islets. Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 9. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human adrenocortical tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 9. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human adrenocortical tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 10. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human placental tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Arrows: Syncytiotrophoblast cells; arrowheads: Capillary endothelia; asterisks: Non-specifically stained erythrocytes. Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 10. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in human placental tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Over- lapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Arrows: Syncytiotrophoblast cells; arrowheads: Capillary endothelia; asterisks: Non-specifically stained erythrocytes. Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 12. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in medullary thyroid cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour. Blue colour represents 4′,6- diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent (C,D,G,H,K,L) enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 12. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in medullary thyroid cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour. Blue colour represents 4′,6- diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent (C,D,G,H,K,L) enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Figure 13. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in renal clear cell cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Journal: International journal of molecular sciences

    Article Title: Expression of the Calcitonin Receptor-like Receptor (CALCRL) in Normal and Neoplastic Tissues.

    doi: 10.3390/ijms24043960

    Figure Lengend Snippet: Figure 13. Double-labelling immunohistochemical analysis of calcitonin receptor-like receptor (CAL- CRL) expression (A,E,I) and the expression of receptor activity-modifying protein (RAMP) 1 (B), RAMP2 (F), or RAMP3 (J) in renal clear cell cancer tissue. Labelling of CALCRL was visualised using Cy3-conjugated anti-rabbit antibody (red). Labelling of RAMP1, RAMP2, or RAMP3 was visualised using Alexa Fluor 488-conjugated rabbit anti-RAMP1, -RAMP2, or -RAMP3 antibody (green). Overlapping expression is represented by orange/yellow colour (C,D,G,H,K,L). Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. (D,H,L) represent enlarged sections of (C,G,K). Scale bar: 100 µm (A–C,E–G,I–K), 50 µm (D,H,L).

    Article Snippet: The cells were then washed thoroughly with phosphate-buffered saline and incubated overnight at 4 ◦C with Alexa 488-conjugated rabbit polyclonal anti-RAMP1, RAMP2, or RAMP3 antibody (1:100 dilution; Bioss Antibodies, Woburn, MA, USA; catalogue numbers, bs-1567R-A488; bs-11971R-A488; bs-11972R-A488; the antibodies were tested beforehand for specificity by means of siRNA knockdown experiments in BON-1 cells, which (as could also be shown in the present paper) endogenously express all three RAMP proteins [siRNAs used: RAMP1, sc-40894; RAMP2, sc-3678; RAMP3, sc-40896; Santa Cruz Biotechnology; Supplemental Figure S7] and by means of peptide neutralisations in RAMPpositive tissues (duodenum; placenta); Supplemental Figure S8).

    Techniques: Immunohistochemical staining, Expressing, Activity Assay, Staining

    Western blotting of CGRP-related proteins. (a) Western blotting and (b)–(e) quantitative analysis indicated that CGRP promoted the protein contents of CALCRL, RAMP-1, pPKA/PKA, and pCREB/CREB. ∗ p < 0.05, # p < 0.01, as compared with control and CGRP-BIBN groups ( n = 6).

    Journal: BioMed Research International

    Article Title: Neural Peptide α -CGRP Coregulated Angiogenesis and Osteogenesis via Promoting the Cross-Talk between Mesenchymal Stem Cells and Endothelial Cells

    doi: 10.1155/2022/1585840

    Figure Lengend Snippet: Western blotting of CGRP-related proteins. (a) Western blotting and (b)–(e) quantitative analysis indicated that CGRP promoted the protein contents of CALCRL, RAMP-1, pPKA/PKA, and pCREB/CREB. ∗ p < 0.05, # p < 0.01, as compared with control and CGRP-BIBN groups ( n = 6).

    Article Snippet: Rabbit monoclonal anti-CALCRL (calcitonin receptor-like receptor, 1 : 1000), anti-RAMP-1 (receptor activity modifying protein 1, 1 : 1000), anti-PKA (cAMP-dependent protein kinase, 1 : 1000), anti-pPKA (phospho-PKA, 1 : 1000), anti-CREB (cAMP response element-binding protein, 1 : 1000), anti-pCREB (phospho-CREB, 1 : 1000), and anti-GADPH (glyceraldehyde 3-phosphate dehydrogenase, 1 : 1000) primary antibodies from Cell Signaling Technology (CST, USA) were used.

    Techniques: Western Blot

    (A, B) The graphs show colocalization of receptor activity-modifying protein 1 (RAMP-1, A in green) and calcitonin gene-related peptide (CGRP) (B, in red) on cutaneous nerves by double-labeling immunofluorescent staining. (C) CGRP 8–37 , a CGRP antagonist, was administrated by intraplantar injections into the bilateral hindpaws. Withdrawal latencies on the hot-plate test were measured at 0.5, 1, 2, 4, 6, 8, and 24 h after antagonism. Thermal hypoalgesia was induced after antagonism in a dose-dependent manner. Hot-plate latencies of the CGRP 8–37 antagonism group vs. the saline group were analyzed with two-way repeated measures ANOVA followed by Bonferroni’s post-hoc test. * p <0.05, ** p <0.01, *** p <0.001, represented as CGRP 8–37 (13 nmole/paw, n = 5) group compared to the saline group. # p <0.05, ## p <0.01, represented as CGRP 8–37 (1.3 nmole/paw, n = 5) group compared to the saline group. Bar, 25 µm.

    Journal: PLoS ONE

    Article Title: Role of Peptidergic Nerve Terminals in the Skin: Reversal of Thermal Sensation by Calcitonin Gene-Related Peptide in TRPV1-Depleted Neuropathy

    doi: 10.1371/journal.pone.0050805

    Figure Lengend Snippet: (A, B) The graphs show colocalization of receptor activity-modifying protein 1 (RAMP-1, A in green) and calcitonin gene-related peptide (CGRP) (B, in red) on cutaneous nerves by double-labeling immunofluorescent staining. (C) CGRP 8–37 , a CGRP antagonist, was administrated by intraplantar injections into the bilateral hindpaws. Withdrawal latencies on the hot-plate test were measured at 0.5, 1, 2, 4, 6, 8, and 24 h after antagonism. Thermal hypoalgesia was induced after antagonism in a dose-dependent manner. Hot-plate latencies of the CGRP 8–37 antagonism group vs. the saline group were analyzed with two-way repeated measures ANOVA followed by Bonferroni’s post-hoc test. * p <0.05, ** p <0.01, *** p <0.001, represented as CGRP 8–37 (13 nmole/paw, n = 5) group compared to the saline group. # p <0.05, ## p <0.01, represented as CGRP 8–37 (1.3 nmole/paw, n = 5) group compared to the saline group. Bar, 25 µm.

    Article Snippet: Primary antisera included anti-protein gene product 9.5 (PGP9.5, rabbit, 1∶2000, UltraClone, Isle of Wight, UK), SP (rabbit, 1∶2000, ImmunoStar, Hudson, WI), CGRP (rabbit, 1∶2000, Sigma), TRPV1 (goat, 1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA), and receptor activity-modifying protein-1 (RAMP-1, goat, 1∶1000, Santa Cruz).

    Techniques: Activity Assay, Labeling, Staining, Hot Plate Test

    BMCs was pretreated with PKA inhibitor rAMP (PKAin; 10 µM) for 30 min, then cells were treated with PGE2 (1 µM) for 24 hr. Total mRNA was isolated and analyzed by real-time RT-PCR for expression of CD31, vWF, MMP2, MMP9, CYP19 and COX2.

    Journal: PLoS ONE

    Article Title: Prostaglandin E2 Promotes Endothelial Differentiation from Bone Marrow-Derived Cells through AMPK Activation

    doi: 10.1371/journal.pone.0023554

    Figure Lengend Snippet: BMCs was pretreated with PKA inhibitor rAMP (PKAin; 10 µM) for 30 min, then cells were treated with PGE2 (1 µM) for 24 hr. Total mRNA was isolated and analyzed by real-time RT-PCR for expression of CD31, vWF, MMP2, MMP9, CYP19 and COX2.

    Article Snippet: Compound C and protein kinase A (PKA) inhibitor (rAMP) were from Sigma Aldrich (St Louis, MO).

    Techniques: Isolation, Quantitative RT-PCR, Expressing